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Catalog Number | orb500525 |
---|---|
Category | Antibodies |
Description | E2F1 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, WB |
Predicted Reactivity | Bovine, Equine, Gallus, Porcine, Rabbit, Rat, Sheep |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human E2F1 (101-200/437 aa) |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, ICC=1:100, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 46 kDa |
Target | E2F1 |
UniProt ID | Q01094 |
Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Buffer/Preservatives | 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol. |
Alternative names | E2F 1; E2F transcription factor 1; E2F-1; E2f1 E2F Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
ICC analysis of HepG2 cell using E2F1 polyclonal Antibody at 1:100 dilution;DAPI (blue) was used to stain the cell nuclei.
Western blot:Lane 1:Hela (Human) Cell;Lane 2:A673 (Human) Cell;Lane 3:Molt-4 (Human) Cell;Lane 4:U251 (Human) Cell;Primary:Anti-E2F1 at 1:1000 dilution.
ELISA, FC, IF, IHC, WB | |
Human, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
FC, ICC, IHC-P, WB | |
Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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