E2F1 (phospho-Ser364) antibody

• Catalog Number: orb5095
• Category: Tools
• Description: E2F1 (phospho-Ser364) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human E2F1 around the phosphorylation site of Ser364 (MG(p-S)LR)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, ICC=1:100, Flow-Cyt=1ug/Test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 48 kDa
• Target: phospho-E2F1 (Ser364)
• UniProt ID: Q01094
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: E2F1 (phospho S364); E2F1 (phospho Ser364); p-E2F1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry of Molt4 (Black).Primary Antibody (green line): Rabbit Anti-phospho-E2F1 (Ser364) antibody; Dilution:1μg /10^6 cells.

ICC analysis of HepG2 cell using phospho-E2F1 (Ser364) polyclonal Antibody at 1:100 dilution;DAPI (blue) was used to stain the cell nuclei.

IHC-P analysis of rat testis using phospho-E2F1 Ser364 Polyclonal Antibody at 1:200 dilution.

IHC-P analysis of Human colon carcinoma using phospho-E2F1 Ser364 Polyclonal Antibody at 1:400 dilution.

Western blot:Lane 1:Hela (Human) Cell;Lane 2:A673 (Human) Cell;Lane 3:Molt-4 (Human) Cell;Primary:Anti-phospho-E2F1 (Ser364) at 1:1000 dilution.