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Catalog Number | orb1529679 |
---|---|
Category | Antibodies |
Description | E2F1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP |
Reactivity | Human |
Immunogen | between 300 and 350 |
Concentration | 1000 µg/ml |
Dilution range | WB - Not recommended. Use rabbit anti-E2F1 antibody A300-766A.; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 2,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | E2F1 |
UniProt ID | Q01094 |
NCBI | NP_005216.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | PRB-binding protein E2F-1; retinoblastoma-binding Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human E2F1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-E2F1 used at a dilution of 1:1, 000 (1µg/ml).
Detection of human E2F1 by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-E2F1 antibody.
ELISA, FC, IF, IHC, WB | |
Human, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
FC, ICC, IHC-P, WB | |
Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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