E2F1 Antibody

• Catalog Number: orb1529678
• Category: Antibodies
• Description: E2F1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: between 300 and 350
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. Use rabbit anti-E2F1 antibody A300-766A.; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 2,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: E2F1
• UniProt ID: Q01094
• NCBI: NP_005216.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: PRB-binding protein E2F-1; retinoblastoma-binding
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human E2F1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-E2F1 used at a dilution of 1:1, 000 (1µg/ml).

Detection of human E2F1 by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-E2F1 antibody.