E2F1 Antibody

• Catalog Number: orb1263116
• Category: Antibodies
• Description: E2F1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This E2F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-371 amino acids from human E2F1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 47 kDa
• Target: E2F1
• UniProt ID: Q01094
• NCBI: Q01094
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Transcription factor E2F1, E2F-1, PBR3, Retinoblas
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution Lane 1: NIH-3T3 whole cell lysates Lane 2: A431 whole cell lysates Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution + Hela whole cell lysates Lysates/proteins at 20 ug per lane.

Western blot analysis of E2F1 Antibody (H357) Pab pre-incubated without (lane 1) and with (lane 2) blocking peptide in 293T cell line lysate.