You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1258309 |
---|---|
Category | Antibodies |
Description | E2F1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | A synthetic peptide of human E2F1 |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:1000IF: 1:20 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 70kDa |
Target | E2F1 |
UniProt ID | Q01094 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | E2F1, E2F-1, RBBP3, RBP3, RBAP1 Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:1000IF: 1:20 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using E2F1 antibody (orb1258309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunofluorescence analysis of C6 cells using E2F1 Polyclonal Antibody (orb1258309) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ELISA, FC, IF, IHC, WB | |
Human, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
FC, ICC, IHC-P, WB | |
Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating