E2-25K Antibody

• Catalog Number: orb1523566
• Category: Antibodies
• Description: E2-25K Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 150 and 200
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: E2-25K
• UniProt ID: P61086
• NCBI: NP_005330.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: ubiquitin-conjugating enzyme E2-25 KDA; ubiquitin-
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse E2-25K by western blot. Samples: Whole cell lysate (50 µg) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-E2-25K antibody orb1523566 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human E2-25K by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-E2-25K antibody orb1523566 used for IP at 6 µg/mg lysate.