UBE2K Antibody

• Catalog Number: orb1264990
• Category: Antibodies
• Description: UBE2K Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This HIP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-139 amino acids from the Central region of human HIP2.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:100For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 22 kDa
• Target: UBE2K
• UniProt ID: P61086
• NCBI: P61086
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Ubiquitin-conjugating enzyme E2 K, Huntingtin-inte
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:100For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded H. small intestine section using HIP2 Antibody. Antibody was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Fluorescent image of Hela cell stained with HIP2 Antibody. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HIP2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). HIP2 immunoreactivity is localized to Cytoplasm significantly.

Western blot analysis in 293, mouse C2C12 cell line lysates (35 ug/lane).