UBE2K Antibody

• Catalog Number: orb1247398
• Category: Antibodies
• Description: UBE2K Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-SWDVETATELLLSN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 24kDa band observed in Jurkat cell lysates (calculated MW of 22.4 kDa according to NP_005330). Recommended for use at 1-3 ug/ml.Immunohistochemistry:In paraffin embedded Human Brain shows pixulate cytoplasm stainng in neurons of the Cortex. Recommended concentration, 1-2ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: UBE2K
• UniProt ID: P61086
• NCBI: NP_005330.1, NP_001104582.1, NP_001104583.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: UBE2K, HIP2, huntingtin interacting protein 2, LIG
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 24kDa band observed in Jurkat cell lysates (calculated MW of 22.4 kDa according to NP_005330). Recommended for use at 1-3 ug/ml.Immunohistochemistry:In paraffin embedded Human Brain shows pixulate cytoplasm stainng in neurons of the Cortex. Recommended concentration, 1-2ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247398 staining (1.5 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb1247398 (3.8 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.