E-cadherin (Extracellular)

• Catalog Number: orb1294382
• Category: Antibodies
• Description: Rabbit polyclonal antibody to E-cadherin antibody (Extracellular domain)
• Target: E-cadherin (Extracellular)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit-IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human E-cadherin
• Tested applications: IF, IHC, WB
• Dilution range: Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:100-1:200
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only

Anti-E cadherin antibody at 1/500 dilution, Lysates/proteins at 60 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294382 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling E-cadherin with orb1294382 at 1/100.

Immunohistochemical analysis of paraffin embedded Human Liver carcinoma tissue labeling E-cadherin with orb1294382 at 1/100.

All lanes: Anti-E cadherin antibody at 1/500 dilution, Lysates/proteins at 60 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294382 overnight at 4°C.

All lanes: Anti-E cadherin antibody at 1/500 dilution, Lysates/proteins at 60 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294382 overnight at 4°C.

Immunofluorescence of paraffin-embedded sections. Cell: mouse colon; Green: E-cadherin (orb1294382): 1-200; Anti-rabbit 488: 1-300.