DIAPH1 antibody

• Catalog Number: orb556010
• Category: Antibodies
• Description: Rabbit polyclonal antibody to DIAPH1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human DIAPH1. The exact sequence is proprietary.
• Concentration: 0.69 mg/ml
• Dilution range: WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000
• Form/Appearance: Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• MW: 141
• Target: DIAPH1
• UniProt ID: O60610
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.01% Thimerosal. 0.1M Tris-Glycine (pH7), 10% Glycerol
• Alternative names: diaphanous related formin 1 , DFNA1 , DIA1 , DRF1
• Note: For research use only
• Application notes: Positive control: H1299 , Raji
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells using DIAPH1 antibody

Immunohistochemical staining of human gastric tissue using DIAPH1 antibody

Western blot analysis of H1299(Lane 1), Raji(Lane 2) using DIAPH1 antibody

Western blot analysis of Raji cells using HLA-DR beta antibody