DIAPH1 antibody

• Catalog Number: orb340970
• Category: Antibodies
• Description: Rabbit polyclonal antibody to DIAPH1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse
• Immunogen: Recombinant full length protein of human Dia 1
• Dilution range: WB: 1:500-1:2000, IHC-P: 1:50-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: DIAPH1
• Entrez: 1729, 13367
• UniProt ID: O08808, O60610
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: DIAP1; Protein diaphanous homolog 1; Diaphanous-re
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Dia 1 expression in MCF7 (A), HepG2 (B), mouse liver (C), mouse lung (D) whole cell lysates. (Predicted band size: 141 kD; Observed band size: 150 kD)

Immunofluorescent analysis of Dia 1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).