DIAPH1 antibody

• Catalog Number: orb20452
• Category: Antibodies
• Description: Goat polyclonal antibody to DIAPH1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Mouse
• Dilution range: ELISA: 1:32000, WB: 0.3-1 μg/ml
• Conjugation: Unconjugated
• MW: 141; 140
• Target: DIAPH1
• Entrez: 1729
• Protein Sequence: QTKTSKAKKDQ
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti DIAPH1 antibody, anti diaphanous homolog 1 (D
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 140kDa band observed in lysates of cell line NIH3T3 (calculated MW of 141kDa according to Human NP_005210.3 and 139kDa according to Mouse NP_031884.1). Recommended concentration: 0.3-1 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb20452 (0.3ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.