Desmoglein 3 Antibody / DSG3

• Catalog Number: orb385987
• Category: Antibodies
• Description: Recognizes a protein of 130kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members are identified and all are members of the cadherin cell adhesion molecule superfamily.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant human protein corresponding to the extracellular portion of DSG3 was used as the immunogen for the Desmoglein 3 antibody.
• UniProt ID: P32926
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Titering of the CD20 antibody may be required for optimal performance.
• Antibody Type: Primary Antibody
• Clone Number: 5G11
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Desmoglein 3 antibody is available for research use only.
• Note: For research use only

IHC testing of FFPE human skin with Desmoglein 3 antibody (clone 5G11). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free Desmoglein 3 Antibody (clone 5G11). Confirmation of Integrity and Purity of the Antibody.