Desmin Antibody

• Catalog Number: orb196275
• Category: Antibodies
• Description: Desmin Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Desmin recombinant protein (Position: M1-T304). Human Desmin shares 97% amino acid (aa) sequence identity with both mouse and rat Desmin.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat, -Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 53536 MW
• UniProt ID: P17661
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Desmin;DES;
• Note: For research use only
• Application notes: WB: The detection limit for Desmin is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of Desmin using anti-Desmin antibody.Lane 1:recombinant human Desmin protein 0.5ng.

WB analysis of Desmin using anti-Desmin antibody.Lane 1:rat skeletal muscle Tissue;2:rat cardiac muscleTissue;3:mouse skeletal muscle tissue;4:mouse Cardiac MuscleTissue;5:HELA Cell;6:HT1080 Cell;7:COLO320 Cell;8:HEPA Cell;9:NIH3T3 Cell.

IF analysis of Desmin using anti-Desmin antibody. Desmin was detected in a paraffin-embedded section of mouse skeletal muscle tissue.

IF analysis of Desmin using anti-Desmin antibody. Desmin was detected in a paraffin-embedded section of mouse heart tissue.

IF analysis of Desmin using anti-Desmin antibody. Desmin was detected in a paraffin-embedded section of rat skeletal muscle tissue.

IF analysis of Desmin using anti-Desmin antibody. Desmin was detected in a paraffin-embedded section of rat heart tissue.

IHC analysis of Desmin using anti-Desmin antibody.Desmin was detected in frozen section of rat cardiac muscle tissues.

IHC analysis of Desmin using anti-Desmin antibody.Desmin was detected in frozen section of mouse cardiac muscle tissues.

IHC analysis of Desmin using anti-Desmin antibody.Desmin was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of Desmin using anti-Desmin antibody.Desmin was detected in paraffin-embedded section of mouse skeletal muscle tissues.

IHC analysis of Desmin using anti-Desmin antibody.Desmin was detected in paraffin-embedded section of rat cardiac muscle tissues.