DES Antibody

• Catalog Number: orb1246609
• Category: Antibodies
• Description: DES Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: The immunogen for this antibody is: C-RDGEVVSEATQQQHE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 55-60kDa band observed in Human, Mouse, Rat and Pig Skeletal Muscle lysates (calculated MW of 53.5kDa according to Human NP_001918.3, Mouse NP_034173.1, Rat NP_071976.1 and 53.6kDa according to Pig NP_001001535.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Heart and Skeletal Muscle. Recommended concentration: 5ug/ml. In paraffin embedded Human Heart, expression of Desmin is detected in myocardial muscle and not in adjacent vascular smooth muscle. Recommended concentration:3.75ug/ml. Data obtained from previous batch.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: DES
• UniProt ID: P17661
• NCBI: NP_001918.3
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: DES, desmin, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 55-60kDa band observed in Human, Mouse, Rat and Pig Skeletal Muscle lysates (calculated MW of 53.5kDa according to Human NP_001918.3, Mouse NP_034173.1, Rat NP_071976.1 and 53.6kDa according to Pig NP_001001535.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Heart and Skeletal Muscle. Recommended concentration: 5ug/ml. In paraffin embedded Human Heart, expression of Desmin is detected in myocardial muscle and not in adjacent vascular smooth muscle. Recommended concentration:3.75ug/ml. Data obtained from previous batch.
• Expiration Date: 12 months from date of receipt.

orb1246609 (1 ug/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Skeletal Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1246609 (5 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Data obtained from previous batch.

orb1246609 (5 ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246609 (3.75 ug/ml) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

orb1246609 Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.