Desmin, monoclonal Antibody

• Catalog Number: orb1294251
• Category: Antibodies
• Description: Mouse Monoclonal antibody to Desmin
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Mouse IgG2a
• Dilution range: Western Blot 1:500-1:1000 Immunofluorescence 1:100-1:200 Immunohistochemistry (Paraffin) 1:50-1:100
• Conjugation: Unconjugated
• Target: Desmin, monoclonal
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-Desmin antibody at 1/1000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 10% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294251 overnight at 4°C.

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294251 anti-Desmin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Anti-Desmin antibody at 1/1000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 10% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294251 overnight at 4°C. Troponin I, cardiac muscle (orb1294291). Troponin T, cardiac muscle (orb1294292).

Desmin monoclonal antibody orb1294251. Immunoperoxidase staining of formalin fixed, paraffin-embedded human smooth muscle tissue.