DDX4/MVH Antibody

• Catalog Number: orb97089
• Category: Antibodies
• Description: DDX4/MVH Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human DDX4 (253-272aa EDEDSIFAHYQTGINFDKYD), identical to the the related rat and mouse sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat Immunocytochemistry , 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 79308 MW
• UniProt ID: Q9NQI0
• Sensitivity: < 10pg/ml
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Probable ATP-dependent RNA helicase DDX4;3.6.4.13;
• Note: For research use only
• Application notes: WB: The detection limit for DDX4 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Confocal immunofluoroscence analysis of MCF-7 cells using DDX4 antibody

Western blot analysis of Lane 1: Rat Testis Tissue Lysate, Lane 2: Mouse Testis Tissue Lysate, Lane 3: HELA Cell Lysate using DDX4 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Ovary Tissue using DDX4 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Testis Tissue using DDX4 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Intestine Tissue using DDX4 antibody