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    DDX4/MVH Antibody (monoclonal, 3C3)

    Catalog Number: orb865594

    DispatchUsually dispatched within 5-10 working days
    $ 520.00
    Catalog Numberorb865594
    CategoryAntibodies
    DescriptionDDX4/MVH Antibody (monoclonal, 3C3)
    Species/HostMouse
    ClonalityMonoclonal
    Clone Number3C3
    Tested applicationsIF, IHC, WB
    ReactivityMouse, Rat
    IsotypeMouse IgG2b
    ImmunogenE.coli-derived human DDX4/MVH recombinant protein (Position: D3-D666).
    ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
    Dilution rangeWestern blot, 0.25-0.5 μg/ml, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat Immunofluorescence, 5 μg/ml, Mouse, Rat
    Form/AppearanceLyophilized
    ConjugationUnconjugated
    MW79 kDa
    UniProt IDQ9NQI0
    StorageAt -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
    NoteFor research use only
    Application notesTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
    Expiration Date12 months from date of receipt.
    DDX4/MVH Antibody (monoclonal, 3C3)

    Western blot analysis of DDX4/MVH using anti-DDX4/MVH antibody (orb865594). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-DDX4/MVH antigen affinity purified monoclonal antibody (Catalog # orb865594) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90502) with Tanon 5200 system. A specific band was detected for DDX4/MVH at approximately 79 kDa. The expected band size for DDX4/MVH is at 79 kDa.

    DDX4/MVH Antibody (monoclonal, 3C3)

    IHC analysis of DDX4/MVH using anti-DDX4/MVH antibody (orb865594). DDX4/MVH was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-DDX4/MVH Antibody (orb865594) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90443) with DAB as the chromogen.

    DDX4/MVH Antibody (monoclonal, 3C3)

    IHC analysis of DDX4/MVH using anti-DDX4/MVH antibody (orb865594). DDX4/MVH was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-DDX4/MVH Antibody (orb865594) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90443) with DAB as the chromogen.

    DDX4/MVH Antibody (monoclonal, 3C3)

    IF analysis of DDX4/MVH using anti-DDX4/MVH antibody (orb865594). DDX4/MVH was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-DDX4/MVH Antibody (orb865594) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (orb1474936) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

    DDX4/MVH Antibody (monoclonal, 3C3)

    IF analysis of DDX4/MVH using anti-DDX4/MVH antibody (orb865594). DDX4/MVH was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-DDX4/MVH Antibody (orb865594) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (orb1474936) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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