DDX41 Antibody

• Catalog Number: orb2636901
• Category: Antibodies
• Description: DDX41 (Probable ATP-dependent RNA helicase DDX41, DEAD box protein abstrakt homolog) is a 622 amino acid protein encoded by the human gene DDX41. DDX41 belongs to the DEAD box helicase family (DDX41 subfamily) and contains one CCHC-type zinc finger, one helicase ATP-binding domain and one helicase C-terminal domain. DDX41 is required during post-transcriptional gene expression and is thought to be involved in pre-mRNA splicing. DDX41 is believed to be a probable ATP-dependent RNA helicase. RNA helicases are highly conserved enzymes that utilize the energy derived from NTP hydrolysis to modulate the structure of RNA. RNA helicases participate in all biological processes that involve RNA, including transcription, splicing and translation.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-DDX41-1B4
• Tested applications: FACS, IF, WB
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: Recombinant full-length human DDX41 protein was used as the immunogen for the DDX41 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This DDX41 antibody is available for research use only.
• UniProt ID: Q9UJV9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the DDX41 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using DDX41 antibody (clone PCRP-DDX41-1B4). These results demonstrate the foremost specificity of the PCRP-DDX41-1B4 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

FACS staining of PFA-fixed human HeLa cells with DDX41 antibody (blue, clone PCRP-DDX41-1B4), and unstained cells (red).

Immunofluorescent staining of PFA-fixed human MCF-7 cells using DDX41 antibody (green, clone PCRP-DDX41-1B4) and phalloidin (red).

SDS-PAGE analysis of purified, BSA-free DDX41 antibody (clone PCRP-DDX41-1B4) as confirmation of integrity and purity.