DDX1 Antibody

• Catalog Number: orb614109
• Category: Antibodies
• Description: DDX1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human DDX1 recombinant protein (Position: K562-F740).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: "Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human"
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 88 kDa
• UniProt ID: Q92499
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: ATP-dependent RNA helicase DDX1; DEAD box protein
• Note: For research use only
• Application notes: "Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users." . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-DDX1 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.

IF analysis of DDX1 using anti-DDX1 antibody.

IHC analysis of DDX1 using anti-DDX1 antibody.

IHC analysis of DDX1 using anti-DDX1 antibody.

IHC analysis of DDX1 using anti-DDX1 antibody.

IHC analysis of DDX1 using anti-DDX1 antibody.

WB analysis using anti-DDX1 antibody.Lane 1:rat skeletal muscle tissue, Lane 2:rat kidney tissue, Lane 3:mouse heart tissue.

WB analysis using anti-DDX1 antibody.Lane 1:K562 cell, Lane 2:Caco-2 cell, Lane 3:U-87MG cell, Lane 4:A549 cell, Lane 5:HeLa cell.