DDIT3 Antibody

• Catalog Number: orb1269882
• Category: Antibodies
• Description: DDIT3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, WB
• Predicted Reactivity: Bovine, Hamster
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This DDIT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-149 amino acids from the C-terminal region of human DDIT3.
• Concentration: batch dependent
• Dilution range: For FACS starting dilution is: 1:25For WB starting dilution is: 1:2000For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 19 kDa
• Target: DDIT3
• UniProt ID: P35638
• NCBI: P35638
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: DNA damage-inducible transcript 3 protein, DDIT-3,
• Note: For research use only
• Application notes: For FACS starting dilution is: 1:25For WB starting dilution is: 1:2000For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37oC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (OH191631) at 1/400 dilution for 40 min at 37oC. Isotype control antibody (blue line) was rabbit IgG (1 ug/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.

Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37oC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (OH191631) at 1/400 dilution for 40 min at 37oC. Isotype control antibody (blue line) was rabbit IgG (1 ug/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.

Western Blot at 1:2000 dilution + human skeletal muscle lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution + PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.

Fluorescent image of Hela cell stained with.Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DDIT3 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). DDIT3 immunoreactivity is localized to Cytoplasm significantly.

Western blot analysis in mouse testis tissue lysates (35 ug/lane).This demonstrates the DDIT3 antibody detected the DDIT3 protein (arrow).

Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.