DDB1 Antibody

• Catalog Number: orb312104
• Category: Antibodies
• Description: DDB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Predicted Reactivity: Bovine, Canine, Equine, Hamster, Monkey, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human DDB1 recombinant protein (Position: S1011-H1140). Human DDB1 shares 99.2% amino acid (aa) sequence identity with both mouse and rat DDB1.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 126968 MW
• UniProt ID: Q16531
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: DNA damage-binding protein 1;DDB p127 subunit;DNA
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of 293T cells using anti-DDB1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of DDB1 using anti-DDB1 antibody.Lane 1:human HeLa cell; 2:human HepG2 cell; 3:human HEK293 cell; 4:human K562 cell; 5:human Raji cell; 6:human CACO-2 cell; 7:human THP-1 cell.

WB analysis of DDB1 using anti-DDB1 antibody.Lane 1:rat brain tissue; 2:rat kidney tissue; 3:rat C6 cell; 4:mouse brain tissue; 5:mouse kidney tissue; 6:mouse testis tissue.

IF analysis of DDB1 using anti-DDB1 antibody. DDB1 was detected in immunocytochemical section of A431 cells.

IHC analysis of DDB1 using anti-DDB1 antibody. DDB1 was detected in paraffin-embedded section of Mouse Liver Tissue.

IHC analysis of DDB1 using anti-DDB1 antibody. DDB1 was detected in paraffin-embedded section of Rat Liver Tissue.

IHC analysis of DDB1 using anti-DDB1 antibody. DDB1 was detected in paraffin-embedded section of Human Intestinal Cancer Tissue.

IHC analysis of DDB1 using anti-DDB1 antibody.DDB1 was detected in immunocytochemical section of A549 cell.

IHC analysis of DDB1 using anti-DDB1 antibody.DDB1 was detected in immunocytochemical section of PC-3 cell.

IHC analysis of DDB1 using anti-DDB1 antibody.DDB1 was detected in immunocytochemical section of SMMC-7721 cell.

IHC analysis of DDB1 using anti-DDB1 antibody.DDB1 was detected in frozen section of human placenta tissue.