DDAH1 antibody

• Catalog Number: orb18846
• Category: Antibodies
• Description: Goat polyclonal antibody to DDAH1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Reactivity: Bovine, Human
• Dilution range: ELISA: 1:1000, WB: 0.1-0.3 μg/ml, IF/ICC: 10μg/ml, FACS: 10ug/ml
• Conjugation: Unconjugated
• MW: 31.1; 20.2; 20.5
• Target: DDAH1
• Entrez: 23576
• Protein Sequence: TCCSVLINKKVDS
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti DDAH1 antibody, anti dimethylarginine dimethy
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 37kDa band observed in human kidney, liver and pancreas lysates (calculated MW of 31.1kDa according to NP_036269.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pullamsetti et al, FASEB J. 2005 Jul;19(9):1175-7; PMID: 15827267). Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of paraformaldehyde fixed HeLa cells using DDAH1 antibody.

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells using DDAH1 antibody.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using DDAH1 antibody.

Western blot analysis of Human Cerebellum (A), Kidney (B) and Testes (C) lysate using DDAH1 antibody.

Western blot analysis of HepG2 (A) and LNCaP (B) cell lysate using DDAH1 antibody.