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Catalog Number | orb1520123 |
---|---|
Category | Antibodies |
Description | Cytokeratin Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | ICC, IF, IHC |
Reactivity | Human |
Immunogen | Human epidermal callus |
Concentration | 200 µg/ml |
Dilution range | IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC-IF - 1:100 - 1:500 |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | Cytokeratin |
UniProt ID | P08779, P19012, P08727, P13645, P02533 |
NCBI | NP_002266, NP_005548, NP_000412, NP_002267, NP_000517 |
Storage | 2 - 8°C |
Buffer/Preservatives | Phosphate Buffered Saline (PBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | K10; Keratin-10; keratin, type I cytoskeletal 10; Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human Cytokeratin by immunohistochemistry. Sample: FFPE section of colon carcinoma. Antibody: Mouse anti-Cytokeratin monoclonal antibody [AE1/AE3]. Secondary: HRP-conjugated goat anti-mouse IgG.
Detection of human Cytokeratin by immunocytochemistry. Sample: FFPE section of MCF7 cells. Antibody: Mouse anti-Cytokeratin monoclonal antibody [AE1/AE3] (orb1520123). Secondary: HRP-conjugated goat anti-mouse IgG.
Detection of human Cytokeratin by immunocytochemistry. Sample: acetone/methanol fixed Hela cells. Antibody: Mouse monoclonal anti-Cytokeratin antibody [AE1/AE1] (orb1520123) used at 1:100. Secondary: DyLight® 594-conjugated goat anti-mouse IgG. Counterstain: DAPI.
FC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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