Cytokeratin 18/KRT18 Antibody

• Catalog Number: orb389489
• Category: Antibodies
• Description: Cytokeratin 18/KRT18 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Cytokeratin 18 recombinant protein (Position: E204-H430). Human Cytokeratin 18 shares 87.7% and 85.9% amino acid (aa) sequence identity with mouse and rat Cytokeratin 18, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry(Frozen Section), 0.5-1 μg/ml, Human Immunocytochemistry, 0.5-1 μg/ml, Human Immunofluorescence, 2 μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 48058 MW
• UniProt ID: P05783
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Keratin, type I cytoskeletal 18;Cell proliferation
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-Cytokeratin 18 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of A549 cells using anti-Cytokeratin 18 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Cytokeratin 18 using anti-Cytokeratin 18 antibody.Lane 1:human placenta tissue; 2:human CACO-2 cell; 3:human A549 cell; 4:human A431 cell; 5:rat liver tissue; 6:rat thymus tissue; 7:mouse liver tissue; 8:mouse thymus tissue.

IF analysis of Cytokeratin 18 using anti-Cytokeratin 18 antibody.Cytokeratin 18 was detected in paraffin-embedded section of human intestinal cancer tissues.

IF analysis of Cytokeratin 18 using anti-Cytokeratin 18 antibody.Cytokeratin 18 was detected in paraffin-embedded section of human lung cancer tissues.

IHC analysis of Cytokeratin 18 using anti-Cytokeratin 18 antibody. Cytokeratin 18 was detected in paraffin-embedded section of human placenta tissues.

IHC analysis of Cytokeratin 18 using anti-Cytokeratin 18 antibody. Cytokeratin 18 was detected in paraffin-embedded section of rat kidney tissues.

IHC analysis of Cytokeratin 18 using anti-Cytokeratin 18 antibody. Cytokeratin 18 was detected in paraffin-embedded section of mouse kidney tissues.

IHC analysis of Cytokeratin 18 using anti-Cytokeratin 18 antibody. Cytokeratin 18 was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of Cytokeratin 18 using anti-Cytokeratin 18 antibody. Cytokeratin 18 was detected in paraffin-embedded section of rat intestine tissues.

IHC analysis of Cytokeratin 18 using anti-Cytokeratin 18 antibody. Cytokeratin 18 was detected in paraffin-embedded section of mouse intestine tissues.