Cytokeratin 5 Antibody / KRT5

• Catalog Number: orb1151884
• Category: Antibodies
• Description: Mouse monoclonal antibody to Cytokeratin 5
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids 316-590 was used as the immunogen for the CK5 antibody.
• UniProt ID: P13647
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the CK5 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: KRT5/6466
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This CK5 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human prostate tissue with CK5 antibody (clone KRT5/6466). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free CK5 antibody (KRT5/6466) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using CK5 antibody (clone KRT5/6466). These results demonstrate the foremost specificity of the KRT5/6466 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.