Cytokeratin 5 Antibody / KRT5

• Catalog Number: orb1151790
• Category: Antibodies
• Description: Mouse monoclonal antibody to Cytokeratin 5
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids 316-590 was used as the immunogen for the recombinant Cytokeratin 5 antibody.
• UniProt ID: P13647
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the recombinant Cytokeratin 5 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: rKRT5/6398
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This recombinant Cytokeratin 5 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human squamous cell carcinoma tissue with recombinant Cytokeratin 5 antibody (clone rKRT5/6398). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human prostate tissue with recombinant Cytokeratin 5 antibody (clone rKRT5/6398). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human testis tissue with recombinant Cytokeratin 5 antibody (clone rKRT5/6398) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Cytokeratin 5 antibody (clone rKRT5/6398) as confirmation of integrity and purity.