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Catalog Number | orb1821564 |
---|---|
Category | Antibodies |
Description | Cytokeratin 20 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 10A2 |
Tested applications | ELISA, IHC, WB |
Reactivity | Human |
Isotype | IgG2a |
Immunogen | The original antibody was generated by immunizing female ICR mice with recombinant human CK20 histidine-tagged fusion protein in Freund's complete adjuvant. |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Target | Cytokeratin 20 |
UniProt ID | P35900 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | CK20; CK-20; K20; KRT20; Cytokeratin-20; Keratin-2 Read more... |
Note | For research use only |
Application notes | The binding characterization of this antibody was done using ELISA. The affinity constant of the original IgG2a antibody was determined to be 1.92 × 10^9 M^(-1). This antibody was capable of detecting recombinant CK20 protein in a western blot assay. This antibody is similar to commercially available anti-Cytokeratin 20 antibody KS20.8 in staining various human tissues. This antibody was used in the immunohistochemical staining of stomach epithelial tissues and gastric adenocarcinomas (CN113583120). |
Expiration Date | 12 months from date of receipt. |
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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