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Catalog Number | orb1294302 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Cytokeratin 20 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / encompassing a sequence within the center region |
Dilution range | Western Blot 1:1000–1:2000 Immunofluorescence 1:200–1:500 Immunohistochemistry (Paraffin) 1:200–1:300 |
Conjugation | Unconjugated |
Target | Cytokeratin 20, CK20 |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-Cytokeratin 20 antibody - at 1/2000 dilution, Lysates/proteins at 45 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked an hour before being incubated with orb1294302 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 20 with orb1294302 at 1/200.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 20 with orb1294302 at 1/200.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294302 anti-Cytokeratin 20 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294302 anti-Cytokeratin 20 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IHC-P, WB | |
Mouse | |
Human, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
FC, ICC, IF, IHC, IP, WB | |
Human, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
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