Cytokeratin 20 antibody

• Catalog Number: orb556706
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Cytokeratin 20
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Cytokeratin 20. The exact sequence is proprietary.
• Concentration:

  1 mg/ml

• Dilution range: WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000
• Form/Appearance: Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• MW: 48 kDa
• Target: Cytokeratin 20
• UniProt ID: P35900
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.025% ProClin 300. 1XPBS (pH7), 20% Glycerol
• Alternative names: keratin 20 , CD20 , CK-20 , CK20 , K20 , KRT21
• Note: For research use only
• Application notes: Positive control: HeLa
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells using Cytokeratin 20 antibody

Immunohistochemical staining of mouse urinary bladder using Cytokeratin 20 antibody

Immunohistochemical staining of HT29 xenograft using Cytokeratin 20 antibody

Western blot analysis of HeLa cells using Cytokeratin 20 antibody