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Product Overview
Product Name Cytokeratin 20 antibody
Catalog Number orb10405
Species/HostRabbit
ReactivityHuman, Mouse, Rat
ConjugationUnconjugated
Tested applicationsELISA, IF, IHC-P, WB
Immunogen KLH conjugated synthetic peptide derived between 161-240 amino acids of human Cytokeratin 20
Target Cytokeratin 20
Alternative Names
Product Properties
Form/Appearance Liquid: 0.01M TBS (pH7.4), 0.5% BSA, 0.015% ProClin™ 300 and 25% glycerol
Concentration - 100 μg (in 200 μl): 0.5 mg/ml
- 200 μg (in 400 μl): 0.5 mg/ml
Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Isotype IgG
ClonalityPolyclonal
Purity Affinity purified by Protein A
MW 48 kDa
Uniprot ID P35900 (Human); Q9D312 (Mouse); P25030 (Rat)
Entrez 54474
Product Description

Rabbit polyclonal to Cytokeratin 20

Application Notes
Dilution Range WB: 1:500-2000, ELISA: 1:500-1000, IHC-P: 1:400-800, IF: 1:100-500
Validation Images
Paraffin-embedded mouse stomach tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). Cytokeratin 20 antibody at 1:200 dilution with overnight incubation at 4 degre
Paraffin-embedded mouse stomach tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). Cytokeratin 20 antibody at 1:200 dilution with overnight incubation at 4 degre
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