Cytokeratin 18 Antibody

• Catalog Number: orb750222
• Category: Antibodies
• Description: This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM510
• Tested applications: FACS, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Human breast cancer PMC 42 cells were used as the immunogen for this anti-Cytokeratin 18 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This anti-Cytokeratin 18 antibody is available for research use only.
• UniProt ID: P05783
• Storage: Store the anti-Cytokeratin 18 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Flow cytometry testing of PFA-fixed human HeLa cells with anti-Cytokeratin 18 antibody (clone SPM510); Red=isotype control, Blue= anti-Cytokeratin 18 antibody.

IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with anti-Cytokeratin 18 antibody (clone SPM510).