Cytokeratin 14 Antibody

• Catalog Number: orb1806434
• Category: Antibodies
• Description: Cytokeratin 14 Antibody
• Species/Host: Mouse
• Clonality: Recombinant
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human
• Immunogen: Human Cytokeratin 14 (C terminal)
• Concentration: 100 µg/ml
• Dilution range: WB: 1:1000, IP: 20 µl/mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Cytokeratin 14
• UniProt ID: P02533
• Storage: Shelf life: 1 year from date of receipt. Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: EBS4; NFJ; Keratin-14; keratin, type I cytoskeleta
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Cytokeratin 14 by western blot. Samples: Whole cell lysate (25 µg) from A-431, HCT 116, MCF-10A, MCF-7, and HaCaT cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Cytokeratin 14 recombinant monoclonal antibody [LL002] (orb1806434) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human Cytokeratin 14 by immunohistochemistry. Sample: FFPE section of bladder carcinoma. Antibody: Mouse anti-Cytokeratin 14 recombinant monoclonal antibody [LL002] (orb1806434). Secondary: HRP-conjugated goat anti-mouse IgG.

Detection of human Cytokeratin 14 (shaded) in FaDu cells by flow cytometry. Antibody: Mouse anti-Cytokeratin 14 recombinant monoclonal antibody [LL002] (orb1806434) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-mouse IgG.

Detection of human Cytokeratin 14 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from MCF-10A cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Cytokeratin 14 recombinant monoclonal antibody [LL002] (orb1806434) used for IP at 20 µl/mg lysate.

Detection of human Cytokeratin 14 by immunocytochemistry. Sample: FFPE section of CAL-27 cells. Antibody: Mouse anti-Cytokeratin 14 recombinant monoclonal antibody [LL002] (orb1806434). Secondary:HRP-conjugated goat anti-mouse IgG.