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Catalog Number | orb1806432 |
---|---|
Category | Antibodies |
Description | Cytokeratin 14 Antibody |
Species/Host | Mouse |
Clonality | Recombinant |
Tested applications | FC, ICC, IHC, IP, WB |
Reactivity | Human |
Immunogen | Human Cytokeratin 14 (C terminal) |
Concentration | 100 µg/ml |
Dilution range | WB: 1:1000, IP: 20 µl/mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | Cytokeratin 14 |
UniProt ID | P02533 |
Storage | Shelf life: 1 year from date of receipt. Storage: 2 - 8°C |
Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | EBS4; NFJ; Keratin-14; keratin, type I cytoskeleta Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human Cytokeratin 14 by western blot. Samples: Whole cell lysate (25 µg) from A-431, HCT 116, MCF-10A, MCF-7, and HaCaT cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Cytokeratin 14 recombinant monoclonal antibody [LL002] (orb1806432) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human Cytokeratin 14 by immunohistochemistry. Sample: FFPE section of bladder carcinoma. Antibody: Mouse anti-Cytokeratin 14 recombinant monoclonal antibody [LL002] (orb1806432). Secondary: HRP-conjugated goat anti-mouse IgG.
Detection of human Cytokeratin 14 (shaded) in FaDu cells by flow cytometry. Antibody: Mouse anti-Cytokeratin 14 recombinant monoclonal antibody [LL002] (orb1806432) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-mouse IgG.
Detection of human Cytokeratin 14 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from MCF-10A cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Cytokeratin 14 recombinant monoclonal antibody [LL002] (orb1806432) used for IP at 20 µl/mg lysate.
Detection of human Cytokeratin 14 by immunocytochemistry. Sample: FFPE section of CAL-27 cells. Antibody: Mouse anti-Cytokeratin 14 recombinant monoclonal antibody [LL002] (orb1806432). Secondary:HRP-conjugated goat anti-mouse IgG.
FC, ICC, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IF, IHC-Fr, IHC-P | |
Bovine, Canine, Equine |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
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