Cyclin E2 antibody

• Catalog Number: orb345568
• Category: Antibodies
• Description: Cyclin E2 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids at the carboxyl terminus of the Cyclin E2 protein.
• Concentration: 1.17 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:85,000, IP: 1:100, WB: 1:200 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity purified antibody is directed against mouse Cyclin E2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Cyclin E2 protein from human based on 100% homology with the immunizing sequence. Cross-reactivity with Cyclin E2 from rat is also predicted based on a 91% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: Q9Z238
• NCBI: 3769612
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Cyclin E2 antibody, G1/S-specific cycl
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kDa in size corresponding to Cyclin E2 protein by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Skp2-/- MEF cells using Cyclin E2 antibody