UBE2C antibody

• Catalog Number: orb18433
• Category: Antibodies
• Description: Goat polyclonal antibody to UBE2C
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IP, WB
• Reactivity: Human
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 10ug/ml
• Conjugation: Unconjugated
• MW: 19.7; 17.4; 16.7; 15.8; 14.3
• Target: UBE2C / UBCH10
• Entrez: 11065
• Protein Sequence: QETYSKQVTSQEP
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti UBE2C antibody, anti UBCH10 antibody, anti dJ
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approximately 20KDa band was detected in mitotic (i.e Nocodazole arrested) HeLa whole cell lysates Recommended for use at 1-3 μg/ml. Immunoprecipitation: 20KDa band precipitated from mitotic HeLa whole cell lysates using protein-G dynabeads. Immunofluorescence: Stained mitotic and late G2 Hela cells, with very faint or no signal in G1 and S phase cels (fixed using paraformaldehyde, primary concentration 2.5 μg/ml, using FITC anti-Goat)
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of U2OS cells using UBE2C antibody.

Immunofluorescence analysis of MCF7 cells using UBE2C antibody.

Western blot analysis of HEK293 (A) and HeLa (B) cell lysate using UBE2C antibody.

Flow cytometric analysis of HeLa cells using UBE2C antibody.