UBE2C Antibody

• Catalog Number: orb1246818
• Category: Antibodies
• Description: UBE2C Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IP, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-QETYSKQVTSQEP
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx.19KDa band observed in lysates of cell lines HeLa and HEK293 (calculated MW of 19.7kDa according to NP_008950.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and MCF7 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. Immunoprecipitation: 20KDa band precipitated from mitotic HeLa whole cell lysates using protein-G dynabeads.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: UBE2C
• UniProt ID: O00762
• NCBI: NP_861516.1, NP_008950.1, NP_861517.1, NP_001268670.1, NP_861518.1, NP_861515.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: UBE2C, UBCH10, dJ447F3.2, ubiquitin-conjugating en
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx.19KDa band observed in lysates of cell lines HeLa and HEK293 (calculated MW of 19.7kDa according to NP_008950.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and MCF7 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. Immunoprecipitation: 20KDa band precipitated from mitotic HeLa whole cell lysates using protein-G dynabeads.
• Expiration Date: 12 months from date of receipt.

orb1246818 (2 ug/ml) staining of HEK293 (A) and HeLa (B) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246818 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic /Plasma Membrane staining.

orb1246818 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.

orb1246818 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml). IgG control: Unimmunized goat IgG (black line).