Cyclin D3/CCND3 Antibody

• Catalog Number: orb196273
• Category: Antibodies
• Description: Cyclin D3/CCND3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Cyclin D3 recombinant protein (Position: Q136-L292). Human Cyclin D3 shares 94% and 93% amino acid (aa) sequences identity with mouse and rat Cyclin D3, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Rat Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 32520 MW
• UniProt ID: P30281
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: G1/S-specific cyclin-D3;CCND3;
• Note: For research use only
• Application notes: WB: The detection limit for Cyclin D3 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of K562 cells using anti-Cyclin D3 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of THP-1 cells using anti-Cyclin D3 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Cyclin D3 using anti-Cyclin D3 antibody.Lane 1:recombinant human Cyclin D3 protein 0.5ng.

WB analysis of Cyclin D4 using anti-Cyclin D4 antibody.Lane 1:rat testis Tissue;2:rat thymus Tissue;3:rat lung Tissue;4:rat ovary Tissue;5:JURKAT Cell;6:A549 Cell;7:MCF-7 Cell;8:HELA Cell.