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    Cyclin D3 CCND3 Rabbit Monoclonal Antibody

    Catalog Number: orb547847

    DispatchUsually dispatched within 5-10 working days
    $ 520.00
    Catalog Numberorb547847
    CategoryAntibodies
    DescriptionCyclin D3 CCND3 Rabbit Monoclonal Antibody
    Species/HostRabbit
    ClonalityMonoclonal
    Clone NumberGID-3
    Tested applicationsFC, ICC, IF, WB
    ReactivityHuman, Mouse
    IsotypeRabbit IgG
    ImmunogenA synthesized peptide derived from human Cyclin D3
    ConcentrationActual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
    Dilution rangeWB 1:1000-1:2000ICC/IF 1:50-1:200FC 1:30
    Form/AppearanceLiquid
    ConjugationUnconjugated
    MW32520 MW
    UniProt IDP30281
    StorageStore at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
    Alternative namesG1/S-specific cyclin-D3;CCND3;
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    NoteFor research use only
    Expiration Date12 months from date of receipt.
    Cyclin D3 CCND3 Rabbit Monoclonal Antibody

    Western blot analysis of Cyclin D3 expression in K562 cell lysate (orb547847). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCND3 monoclonal antibody (Catalog # orb547847) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for CCND3.

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