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Catalog Number | orb1274536 |
---|---|
Category | Antibodies |
Description | CTNNB1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human beta Catenin (NP_001895.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 105kDa |
Target | CTNNB1 |
UniProt ID | P35222 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | CTNNB1, CTNNB, MRD19, armadillo Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using beta Catenin antibody (orb1274536) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Western blot analysis of extracts from normal (control) and beta Catenin knockout (KO) HeLa cells, using beta Catenin antibody (orb1274536) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human tonsil using beta Catenin antibody (orb1274536) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using beta Catenin antibody (orb1274536) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200 ug extracts of U-87MG cells, using 3 ug beta Catenin antibody (orb1274536). Western blot was performed from the immunoprecipitate using beta Catenin antibody (orb1274536) at a dilution of 1:1000.
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC-P | |
Gallus, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC | |
Human, Mouse, Rat | |
Unconjugated |
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