beta Catenin CTNNB1 Antibody (monoclonal, 1F6)

• Catalog Number: orb421109
• Category: Antibodies
• Description: beta Catenin CTNNB1 Antibody (monoclonal, 1F6)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1F6
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1
• Immunogen: E. coli-derived human beta Catenin recombinant protein (Position: A2-K233).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 95 kDa
• UniProt ID: P35222
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Catenin beta-1; Beta-catenin; CTNNB1; CTNNB; OK/SW
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-beta Catenin antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of beta Catenin using anti-beta Catenin antibody.Lane 1:human HeLa cell;2:human COLO-320 cell;3:human HepG2 cell;4:human MCF-7 cell;5:human SW620 cell;6:human 22RV1 cell;7:human Jurkat cell.

IF analysis of beta Catenin using anti-beta Catenin antibody.beta Catenin was detected in immunocytochemical section of A431 cell.

IHC analysis of beta Catenin using anti-beta Catenin antibody.beta Catenin was detected in paraffin-embedded section of human mammary cancer.

IHC analysis of beta Catenin using anti-beta Catenin antibody.beta Catenin was detected in paraffin-embedded section of human mammary cancer.

IHC analysis of beta Catenin using anti-beta Catenin antibody.beta Catenin was detected in immunocytochemical section of A549 cell.