beta Catenin/CTNNB1 Antibody

• Catalog Number: orb402308
• Category: Antibodies
• Description: beta Catenin/CTNNB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human beta Catenin recombinant protein (Position: A2-K233).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Immunofluorescence, 5μg/ml Flow Cytometry, 1-3μg/1x106 cellsbr> Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 95 kDa
• UniProt ID: P35222
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Catenin beta-1; Beta-catenin; CTNNB1; CTNNB; OK/SW
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A549 cells using anti-CTNNB1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis using anti-CTNNB1 antibody.Lane 1:human Hela cell; 2:human MCF-7 cell; 3:human A431 cell; 4:human RT4 cell; 5:human SiHa cell; 6:human T47D cell; 7:rat brain tissue; 8:rat liver tissue; 9:mouse brain tissue; 10:mouse liver tissue.

IF analysis of CTNNB1 using anti-CTNNB1 antibody.CTNNB1 was detected in immunocytochemical section of A431 cell.

IF analysis of CTNNB1 using anti-CTNNB1 antibody. CTNNB1 was detected in a paraffin-embedded section of human intestine cancer tissue.

IHC analysis of CTNNB1 using anti-CTNNB1 antibody.CTNNB1 was detected in paraffin-embedded section of human liver cancer tissue.

IHC analysis of CTNNB1 using anti-CTNNB1 antibody.CTNNB1 was detected in paraffin-embedded section of human prostatic cancer tissue.

IHC analysis of CTNNB1 using anti-CTNNB1 antibody.CTNNB1 was detected in paraffin-embedded section of human tonsil tissue.

IHC analysis of CTNNB1 using anti-CTNNB1 antibody.CTNNB1 was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of CTNNB1 using anti-CTNNB1 antibody.CTNNB1 was detected in paraffin-embedded section of mouse heart tissue.

IHC analysis of CTNNB1 using anti-CTNNB1 antibody.CTNNB1 was detected in paraffin-embedded section of mouse liver tissue.

IHC analysis of CTNNB1 using anti-CTNNB1 antibody.CTNNB1 was detected in paraffin-embedded section of mouse intestine tissue.

IHC analysis of CTNNB1 using anti-CTNNB1 antibody.CTNNB1 was detected in paraffin-embedded section of rat heart tissue.

IHC analysis of CTNNB1 using anti-CTNNB1 antibody.CTNNB1 was detected in paraffin-embedded section of rat liver tissue.

IHC analysis of CTNNB1 using anti-CTNNB1 antibody.CTNNB1 was detected in paraffin-embedded section of rat spleen tissue.

IHC analysis of CTNNB1 using anti-CTNNB1 antibody.CTNNB1 was detected in paraffin-embedded section of rat intestine tissue.