CTNNA1 Antibody

Catalog Number: orb1247702

Catalog Number	orb1247702
Category	Antibodies
Description	CTNNA1 Antibody
Species/Host	Goat
Clonality	Polyclonal
Tested applications	ELISA, IHC, WB
Predicted Reactivity	Canine, Mouse, Rat
Reactivity	Human
Immunogen	The immunogen for this antibody is: C-KREKQDETQTKIK
Concentration	500 ug/mL
Dilution range	Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 100kDa band observed in human colon and human skin lysates (calculated MW of 100kDa according to NP_001894.2). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Prostate and Uterus. Recommended concentration: 3.75ug/ml.
Form/Appearance	Liquid
Conjugation	Unconjugated
Target	CTNNA1
UniProt ID	P35221
NCBI	NP_001894.2
Storage	Aliquot and store at -20°C. Minimize freezing and thawing.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative names	catenin (cadherin-associated protein), alpha 1, 10 Read more...
Note	For research use only
Application notes	Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 100kDa band observed in human colon and human skin lysates (calculated MW of 100kDa according to NP_001894.2). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Prostate and Uterus. Recommended concentration: 3.75ug/ml.
Expiration Date	12 months from date of receipt.

orb1247702 (1 ug/ml) staining of human colon lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing Human CTNNA1 and probed with orb1247702 (mock transfection in first lane).

orb1247702 (3.75 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247702 (3.75 ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.