alpha 1 Catenin/CTNNA1 Antibody

• Catalog Number: orb215892
• Category: Antibodies
• Description: alpha 1 Catenin/CTNNA1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CTNNA1 recombinant protein (Position: D143-D292). Human CTNNA1 shares 98% amino acid (aa) sequence identity with mouse CTNNA1.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 100071 MW
• UniProt ID: P35221
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Catenin alpha-1;Alpha E-catenin;Cadherin-associate
• Note: For research use only
• Application notes: WB: The detection limit for CTNNA1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U-87 cells using anti-CTNNA1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CTNNA1 using anti-CTNNA1 antibody.Lane 1:Recombinant Human CTNNA1 Protein 0.5ng.

WB analysis of CTNNA1 using anti-CTNNA1 antibody.Lane 1:Rat Liver Tissue;2:Rat Lung Tissue;3:Rat Cardiac Muscle Tissue;4:NIH3T3 Cell;5:PC-12 Cell;6:HEPG2 Cell;7:HELA Cell;8:MCF-7 Cell;9:HEPA Cell.

IF analysis of CTNNA1 using anti-CTNNA1 antibody. CTNNA1 was detected in immunocytochemical section of U20S cells.

IHC analysis of CTNNA1 using anti-CTNNA1 antibody.CTNNA1 was detected in paraffin-embedded section of rat intestine tissue.

IHC analysis of CTNNA1 using anti-CTNNA1 antibody.CTNNA1 was detected in paraffin-embedded section of human mammary tissue.

IHC analysis of CTNNA1 using anti-CTNNA1 antibody.CTNNA1 was detected in paraffin-embedded section of mouse intestine tissue.