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Catalog Number | orb1530150 |
---|---|
Category | Antibodies |
Description | CRM1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | between 1025 and C-term |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | CRM1 |
UniProt ID | O14980 |
NCBI | NP_003391.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | CRM1; exportin-1 (required for chromosome region m Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human and mouse CRM1 by western blot.Samples: Whole cell lysate prepared using NETN buffer from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-CRM1 antibody orb1530150 at 0.1 µg/ml.
Detection of mouse CRM1 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:1, 000 (1µg/ml).
Detection of human CRM1 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:5, 000 (0.2µg/ml).
Detection of human CRM1 by western blot of immunoprecipitates.Samples: Whole cell lysate prepared using NETN buffer from HeLa and cells. Antibodies: Affinity purified rabbit anti-CRM1 antibody.
FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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