CRM1 XPO1 Antibody (monoclonal, 5G3)

• Catalog Number: orb421118
• Category: Antibodies
• Description: CRM1 XPO1 Antibody (monoclonal, 5G3)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5G3
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG2b
• Immunogen: E.coli-derived human CRM1 recombinant protein (Position: N966-D1071). Human CRM1 shares 93.4% and 91.5% amino acid (aa) sequence identity with mouse and rat CRM1, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 5 μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 123 kDa
• UniProt ID: O14980
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Exportin-1; Exp1; Chromosome region maintenance 1
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-CRM1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CRM1 using anti-CRM1 antibody.Lane 1:rat liver tissue; 2:rat lung tissue; 3:mouse liver tissue; 4:mouse lung tissue; 5:human HepG2 cell; 6:human SMMC-7721 cell; 7:human HeLa cell; 8:human Jurkat cell.

IF analysis of CRM1 using anti-CRM1 antibody. CRM1 was detected in immunocytochemical section of U20S cells.

IHC analysis of CRM1 using anti-CRM1 antibody.CRM1 was detected in paraffin-embedded section of human lung cancer tissue.

IHC analysis of CRM1 using anti-CRM1 antibody.CRM1 was detected in paraffin-embedded section of human intestinal cancer tissue.