CRM1/XPO1 Antibody

• Catalog Number: orb371700
• Category: Antibodies
• Description: CRM1/XPO1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CRM1 recombinant protein (Position: N966-D1071). Human CRM1 shares 93.4% and 91.5% amino acid (aa) sequence identity with mouse and rat CRM1, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 123386 MW
• UniProt ID: O14980
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Exportin-1;Exp1;Chromosome region maintenance 1 pr
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-CRM1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of U87 cells using anti-CRM1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB:HeLa;COLO-320;MCF-7;A549;A431;SGC-7901;22RV1;rat heart tissue;rat skeletal muscle tissue;rat lung tissue;rat testis tissue;mouse heart tissue;mouse skeletal muscle tissue;mouse lung tissue;mouse testis tissue;mouse ovary tissue.

IF analysis of CRM1 using anti-CRM1 antibody.CRM1 was detected in immunocytochemical section of U20S cell.

IHC analysis of CRM1 using anti-CRM1 antibody.CRM1 was detected in paraffin-embedded section of mouse intestine tissues.

IHC analysis of CRM1 using anti-CRM1 antibody.CRM1 was detected in paraffin-embedded section of rat testis tissues.

IHC analysis of CRM1 using anti-CRM1 antibody.CRM1 was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of CRM1 using anti-CRM1 antibody.CRM1 was detected in frozen section of rat testis tissue.

IHC analysis of CRM1 using anti-CRM1 antibody.CRM1 was detected in frozen section of rat intestine tissue.

IHC analysis of CRM1 using anti-CRM1 antibody.CRM1 was detected in frozen section of mouse intestine tissue.