COX4I1 antibody

• Catalog Number: orb19574
• Category: Antibodies
• Description: Goat polyclonal antibody to COX4I1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:4000, WB: 0.01-0.03 μg/ml, IHC-P: 2.5 μg/ml
• Conjugation: Unconjugated
• MW: 19.6; 15.4
• Target: COX4I1
• Entrez: 1327
• Protein Sequence: QGLASKWDYEKNE
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti COX4I1 antibody, anti cytochrome c oxidase su
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.IHC: In paraffin embedded Human Thyroid Gland shows pixulate (mitochondrial) staining in the cytoplasm of epithelial cells. Recommended concentration, 2-4ug/ml.WB: Approx 18kDa band observed in human skeletal muscle lysates (calculated MW of 19.6kDa according to NP_001852.1). Recommended concentration: 0.01-0.03 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19574 (0.01ug/ml) staining of human muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19574 (2.5ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemical analysis of staining of human muscle lysate using COX4I1 antibody

Western blot analysis of staining of human muscle lysate using COX4I1 antibody