COX4I1 Antibody

• Catalog Number: orb1256931
• Category: Antibodies
• Description: COX4I1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-169 of human COX IV (NP_001852.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 17kDa
• Target: COX4I1
• UniProt ID: P13073
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: COX4I1, COX4, COXIV, MGC72016, COX4-1
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using COX IV Antibody (orb1256931) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Immunohistochemistry of paraffin-embedded rat spleen using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human placenta using COX IV antibody (orb1256931) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using COX IV antibody (orb1256931). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of 22Rv1 cells, using 3 ug COX IV antibody (orb1256931). Western blot was performed from the immunoprecipitate using COX IV antibody (orb1256931) at a dilution of 1:1000.